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背景简介 1948年三月建立了nctc clone 929 (小鼠结缔组织)，细胞系l的克隆。 细胞系l是最早建立的连续培养细胞系之一，而clone 929是最早的克隆株。 从一只100日龄的雄性c3h/an小鼠. Nctc clone 929 细胞是1948年3月从l细胞获得的第一个克隆株，也是在连续培养中建立的第一个细胞株之一。 亲本l细胞来源于100天龄雄性c3h/an小鼠的正常皮下乳晕和脂肪. 细胞按照一定的接种比例传代, 首次按照1:3 进行传代,若细胞在两天内长满可增加传代比例, 若细胞生长三四天还未长满, 可适当缩小传代比例。
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